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The Effect of HFD and Yohimbine Treatment on the Level of Free Sulfide, Sulfane Sulfur, and Bound Sulfane Sulfur in the Rat Liver The level of free sulfide (H 2 S) did not differ significantly between the studied groups, however, a slight but statistically non-significant increase in H 2 S level could be seen in the liver of obese rats without yohimbine treatment when compared to healthy animals (Figure 2A).The level of the total pool of sulfane sulfur was significantly decreased in the liver of HFD-induced obese rats when compared to the liver of healthy animals