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Common clinical indications include lumbar spinal stenosis, disc herniation, radiculopathy, and sciatica
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2022;8(1):e10365
Without efficient escape, released PROTACs may remain trapped within endosomal/lysosomal compartments, thereby limiting their access to cytosolic or nuclear targets and E3 ligases and ultimately attenuating degradation efficacy
After incubation, the beads were washed with 1 ml of ice-cold phosphate-buffered saline (PBS), pH 7.4, three times, and the beads were eluted in sample loading buffer by boiling for 10 min at 100 C
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