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In addition to its participation in the WWC, ascorbate is also required for the regeneration of lipid-soluble antioxidants, particularly tocopherols and tocotrienols (vitamin E), which protect the polyunsaturated fatty acids in the thylakoid membranes from oxidation to chromanoxyl radicals by singlet oxygen

These additives are rarely used in standard research settings where bacteriostatic water is the reconstitution medium

These measures help ensure that researchers receive a formulation with reproducible characteristics suitable for scientific investigation