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Stipanuk MH, Coloso RM, Garcia RAG, Banks MF: Cysteine concentration regulates cysteine metabolism to glutathione, sulfate, and taurine in rat hepatocytes

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On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature

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Inject it into a muscle, preferably in the buttocks

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