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In detail, cFSP1 or hFSP1 from cell lysate supernatant was bound to Ni-Chelating resin in buffer A, and washed with buffer A supplemented with 20 mM imidazole, and an excessive amount of solution containing 2 M potassium bromate and 0.5 M urea until cofactors removal was complete as judged by complete loss of green color and by HPLC

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