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Angiogenesis experiments use tube formation assays in human umbilical vein endothelial cells (HUVECs), matrigel plug assays in mice, and VEGF protein quantification via Western blot to characterize BPC-157's vascular effects

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Most of the work in laboratory animals demonstrates that the positive effects of increased leucine intake or high-protein diets are most pronounced when the animals are also consuming a high-fat diet