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Cyclic AMP response element (CRE)-reporter assay A BHK cell line was stably transfected with the human calcitonin (a) receptor (hCTR a ) and a cAMP responsive element (CRE) luciferase reporter gene

During follicular development, FSH activates the PI3K/AKT/mTOR pathway to inhibit autophagy, promoting granulosa cells proliferation and supporting follicular development (Texada et al., 2019