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First, 1.5 M oligomycin was added to inhibit proton translocation through the F0/F1 complex, thereby blocking ATP synthesis and lowering OCR

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Materials and Methods GSH/GSSG-Glo Assay (Cat.# V6611) CellTox Green Cytotoxicity Assay (Cat.# G8741) menadione (Sigma, Cat.# M5625) l-Buthionine Sulfoximine (Sigma, Cat.# B2515) digitonin (Sigma, Cat.# D141) HEK-293 cells K-562 cells Note: Cell lines were authenticated using STR genotyping ( GenePrint 10 System, Cat.# B9510)

If the molecule is broken down before it enters your system, it loses its unique antioxidant properties

After that, you can decide whether to reintroduce copper peptides at lower frequency or switch to a gentler alternative