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Despite these challenges, CSC-targeted therapies are gradually progressing toward clinical trials, with novel strategies such as pathway inhibitors (Notch, Hedgehog, Wnt, PI3K/AKT/mTOR), immune-based therapies (CAR-T cells and cancer vaccines), and synthetic biology approaches (gene circuits, OVs) showing promising preclinical results

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Salen G, Shefer S, Batta AK, Tint GS, Xu G, Honda A, et al

Decline in glutathione concentrations in intracellular fluids correlate directly with indicators of longevity

However, observational studies cannot establish causation, and confounding factorssuch as differences in overall health status, healthcare engagement, or disease severitymay influence these associations