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30 min pretreatment, 24 h AO exposure) dose-dependently inhibits the release of proinflammatory mediators (TNF-, IL-1, NO) and the expression of proinflammatory proteins (iNOS, COX-2) induced by AO in BV-2 cells, suppresses AO-induced oxidative stress, and restores the antioxidant capacity of cells [5]

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Cells were then incubated with a primary antibody for 12 h at room temperature, followed by the corresponding fluorophore-conjugated secondary antibody (Alexa Fluor) for 45 min and DAPI staining (1 g ml 1 DAPI

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The same logic applies to jaw surgery