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The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 g/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 g/mL) (Bio-Sciences) for 30 min protected from light, at room temperature

A phase 2 randomized controlled trial of personalized peptide vaccine immunotherapy with low-dose dexamethasone versus dexamethasone alone in chemotherapy-naive castration-resistant prostate cancer

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A healthy anus contains two sphincter muscles (internal and external) that work together to control bowel movements, with the internal sphincter relaxing automatically and the external under conscious control

Some people benefit from weekly top-ups, while others maintain their levels with monthly visits