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This result may have been driven by Erysipelatoclostridium and Lactobacillus , the only two microbial genera that significantly differed between the 5A-1MQ-treated and the vehicle-treated mice both switched from WD to LD

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Setting realistic goals: Define specific objectives (pain reduction, return to specific activities, healing verified on imaging) rather than vague "feel better." Understand realistic timeline (weeks for soft tissue, months for bone, months to years for cartilage) preventing premature disappointment

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Solution: Bacteriostatic Water (0.9% Benzyl Alcohol)