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For immunohistochemical staining, the liver tissue sections were blocked with 10% goat serum (Abcam) for 30 min following deparaffinization and antigen retrieval, and then incubated overnight at 4C with mouse-monoclonal CD68 antibody (1:100

This inflammation due to long-term aspartame consumption may decrease reduced and oxidized glutathione levels in the liver

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JPEN J Parenter.Enteral Nutr 1988;12(6):555-562

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