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In addition to MTT assays, we also performed cell counting with a Countess Automated Cell Counter (Applied Biosystems) in experiments where we treated primary melanoma cell lines WM115 and WM1862 with the EPAC inhibitor ESI-09 and transfected with EPAC siRNA

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Existing experience indicates that platforms with many components and highly refined architectures face increasing pressure in process scale-up, quality characterization and safety evaluation

TABLE 1 Microbiota-produced glucoraphanin metabolites in ex vivo studies

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This allows researchers to begin their studies with speed