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These findings are supported by previous evidence from randomized controlled trials, although results remain mixed
The discrepancy between this study and the data herein may reflect the heterogeneous nature of CD and disease severity between the patient donors (the current study used monocytes from individuals with mild-to-moderate CD), the mechanisms of action of the various pro-death stimuli, or differences in apoptotic versus non-apoptotic death in macrophages
[11] Polymerase chain reaction-restriction fragment length polymorphism analyses were used for the genotyping of MTHFR polymorphisms
Then, 3.5 l of the sample was applied onto 45 s glow-discharged quantifoil grids and vitrified in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) at 100% humidity, 7 s blotting time and 10 s waiting time
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