Ten planes of each sample were tracked and four replicates per cell condition were collected with the same parameters and PI intensity of different cell conditions were analyzed with Harmony software (PerkinElmer) using the same settings to optimize the results
The results suggested promotion of PI3K/Akt/FoxO1 signaling [9]
SASA analysis indicated that the solvent-accessible surface area of the GPX3Diosgenin complex ranged from 95 to 110 nm, with no significant change in structural exposure after binding ( Figure 9F )
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