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The BRET signal resulting from ligand stimulation was calculated by subtracting the BRET ratio for the vehicle-treated cells from the ligand-stimulated cells of the same aliquot

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Currently, CRISPR is limited to in vitro and animal studies due to safety and ethical barriers, but advances in delivery vectors and base editing technologies may accelerate clinical translation (Inoue et al., 2023

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As of publication, one of the latest meta-analyses on the topic was published in 2022 and pooled data from all available randomized control trials (RCTs) on liraglutide on obesity regardless of duration [13]