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Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer

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Wash your face and wipe dry

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Thus, the in vivo downregulation of Gsto2 that rescues DA increase, energy metabolism and motor dysfunctions in Trkb Penk -KO mice is significant and previously unappreciated