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The container should be kept in a cool, dry place, away from direct sunlight and heat sources, as exposure to light and warmth can cause the water to degrade over time

There's no strong evidence that a specific hour of the day makes glutathione work better

So experiment

Phys Chem Chem Phys 4:16221627 Tissandier MD, Cowen KA, Feng WY et al (1998) The protons absolute aqueous enthalpy and Gibbs free energy of solvation from cluster-ion solvation data

This corresponds to a time span of about 1.5 s between the first cell-surface contact and the initiation of cell detachment