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Bacteriostatic water should be stored according to the manufacturers instructions and applicable laboratory protocols

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For characterization of cARLA-treated hECs the cells were cultured in 50% PC-conditioned medium on glass bottom chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific) coated with collagen IV and fibronectin at a seeding density of 5 10 4 cells/cm 2

Excessive stress can lead to increased glutamate release and overactivation of NMDAR, resulting in calcium influx and subsequent activation of intracellular signaling pathways that cause neuronal atrophy and death, ultimately leading to mood disorders and depression-like symptoms ( Lycium barbarum polysaccharide (LBP, 25, 50, and 100 mg/kg) to rats for three consecutive weeks intragastrically was found to effectively inhibit the excessive activation of NR2B in the prefrontal cortex of rats, reduce the expression of calmodulin kinase II (CaMKII), a crucial downstream signaling molecule of NMDAR, and alleviate depressive-like behaviors in rats with PTSD ( The natural plant polysaccharides have the ability to modulate neurotransmitters in the brain

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