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Western blotting Briefly, whole proteins were extracted with RIPA Buffer (#89900, Thermo Fisher, Waltham, USA) with protease inhibitor cocktail (#20124ES03, Yeasen, Shanghai, China)

Research Overview GHK-Cu is the copper(II) complex of the tripeptide glycyl-L-histidyl-L-lysine, a naturally occurring human sequence first isolated from plasma by Loren Pickart in 1973 and subsequently identified in saliva and urine

However, approximately half of the patients undergoing endocrine therapy suffer relapses, including mutations in the ER coding gene ESR1 , especially the L536Q and Y537S mutations [6], which present a significant clinical challenge

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Research has linked lower glutathione levels with higher rates of insulin resistance