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and has received consultancy fees from AstraZeneca, Boehringer Ingelheim, Lilly USA, Merck & Co., Inc., Pfizer, Novo Nordisk, Metavant, Afimmune, and Sanofi

and 24 healthy volunteers (12 females, 12 males, mean age 40.5 years, range 3065 years)

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In the future, in-depth analysis of the ASCT2 3D structure using structural biology techniques and an in-depth understanding of the interaction mechanism between ASCT2 and inhibitors will help to optimize the structure of inhibitors and improve their efficacy

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Primary antibodies used included GAPDH (MA5-15738, Thermo Fisher, dilution 1:1000) as a loading control, along with target-specific primary antibodies listed in Table S6