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PLoS Pathog

At a 200-pound body weight, that means 90-145 grams of protein per day, every day, without exception

G.BresE.VrtesiA.FrankZ.PasicsI.EndresJ.et al (2011)

For example, higher levels of homocysteine mean that certain proteins important in cardiovascular disease and neurodegenerative diseases can end up with a homocysteine attached or incorporated into them.[ref] The process of post-translational protein modification happens all the time and in a number of different ways, including the addition of other amino acids, sugar molecules, or fatty acids

Unbound proteins were washed out extensively with the following buffers stepwise: 1% Triton X-100, 500 mM NaCI, 1 mM NEM, 1 M Urea in 1x TBS, 1% Triton X-100, 1 mM NEM in 1x TBS, and 1% Triton X-100 in 1x TBS