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Fab7F38 was affinity-captured by a Protein G Sepharose 4FF column (GE healthcare) and eluted with a low pH elution buffer (100 mM Glycine pH 2.8)

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The IFN- applied cells presented highest p-IRE1 levels by western blotting, followed by NAC, IFN-+NAC, IFN-+NAC + IFN- receptor antagonist, IFN-+ Anta and the control, relatively (Fig