Initially, we explored the use of derivatised phosphines due to their reaction with RSS to incorporate the sulfane sulfur atom 32
Sometimes laboratory evaluation may be appropriate
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While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain
Killed mice were then perfused transcardially with 50 ml of cold phosphate buffered saline (PBS), the meninges were removed, and the brain was blotted dry
