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However, emerging research is now drawing attention to an unexpected issue

Estrogenic and androgenic activity of PCBs, their chlorinated metabolites and other endocrine disruptors estimated with two in vitro yeast assays

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M., Lezamiz, A., Uroz, M., Kosmalska, A

In the second step adding NADPH and glutathione reductase results in conversion of all GSSG to GSH which reacts subsequently with DTNB and fluorescent or colorimetric substrate to be quantified

It is recommended to freeze aliquots at -20C or -80C for extended storage