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Supporting our cytochrome c assays, we confirmed no change in mitochondrial outer membrane permeabilization (MOMP) in cells surviving 5FU treatment (compared to the dimethyl sulfoxide group), and no increase in mitochondrial apoptotic priming based on BH3-profiling 15,16 induced by 5FU treatment (Extended Data Fig

4c, replacement of the first six residues of GLP-2 by those of GLP-1 (i.e., double mutant D 3P E and S 5P T) decreased cAMP accumulation by 12-fold compared to the native peptide, suggesting that subtle substitution may directly alter receptor activation