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Acknowledgments We thank Chih-Shung Wongs research assistants, Ching-Pai Wang and Tsou-Mei Liu, for performing animal drug administration and organ dissection for the studies

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Production of metabolites from amino acids, however, could play an important role in GLP-1 secretion from the colon, as the microbiota can ferment nutrients reaching the distal intestine (mostly colon) and produce metabolites, which may stimulate GLP-1 and PYY secretion [36,37,38]