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[DOI] [PubMed] [Google Scholar] 197.Yu Y., Yang F.-H., Zhang W.-T., Guo Y.-D., Ye L., Yao X.-D

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Protoplasts from barley endosperm A pellet of crude protoplast, obtained at 40-50 g for 2-4 min, was suspended in 2 ml of 40% (w/v) Nycodenz (in 50 mM CaCl2, 25 mM MES, pH 5.5), overlaid with 1 ml each of 26.6%, 13.3% and 6.6% Nycodenz (produced by dilution of the 40% Nycodenz solution with the protoplast medium)