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Two fragment spectra were recorded in the orbitrap analyser (resolution 30000, scan range auto, maximum injection time 100 ms, AGC target 50000) for each precursor, one after collision-induced-dissociation fragmentation and one after higher-energy collisional dissociation type fragmentation

In addition, butyrate can activate the signaling pathway mediated by G protein-coupled receptors (GPRs), further inhibiting the downstream MEK-ERK and NF-B pathways

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