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Cells were cultured in DMEM (Gibco, C11885500) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin, and maintained in a humidified incubator at 37 C with 5% CO 2

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Microcurrent transcutaneous electric nerve stimulation in painful diabetic neuropathy: a randomized placebo-controlled study