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If blood is dropped on a piece of white gauze, it will remain brown as it dries (in contrast, normal deoxygenated blood will absorb oxygen and turn red)

Molecular analysis RNA extraction Total RNA was extracted from tissue samples as previously described (30)

K., Liu, B., Cui, X., Brown, K

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Combining semaglutide with SGLT2 inhibitors, DPP-4 inhibitors, or other glucose-regulating compounds creates synergistic effects that may provide superior glycemic control with potentially reduced side effects

MS-based proteomics For proteomics, extracted and immunoprecipitated proteins from the cecum, liver, and brain were separated on a Bolt 412% Bis-Tris Plus gel (Life Technologies Corporation - Thermo Fisher Scientific Inc., Waltham, MA, USA) for in-gel digestion