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The samples were centrifuged again at 15,000 g at 4C for 30 min

Treatments were separated by one-week washout periods

doi: 10.1073/pnas.1710519114 232 MoudgilKDChoubeyD

151 However, either excessive activation or inhibition of MQC can cause the accumulation of oxidized proteins, expedite abnormal energy metabolism, and induce mitochondrial dysfunction to stimulate aging