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To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit

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10.1016/j.cellbi.2007.04.009 10

Somatostatin has multiple effects on anterior pituitary as well as pancreatic, liver and gastrointestinal function: It inhibits GH secretion indirectly via antagonizing GHRH secretion

Am J Sports Med 2016;44:2622-2628