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LepRb-IRS-PI3K-AKT-FoxO1 FoxO1 is a transcription factor involved in regulation of gluconeogenesis, glycogenolysis as well as adipogenesis

MAPK, Mitogen-activated protein kinase

Cells were then washed once more in PAB and finally resuspended in 300 L of FACS buffer for flow cytometry analysis using (BD LSRFortessa X-20 cell analyzer)

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Both sterile water and saline lack any preservative