2.3 Cell culture and treatment The human normal hepatic cell line LO2 (Shanghai Institute of Biological Sciences, Chinese Academy of Science, Shanghai, China) was maintained in DMEM(BI,United States) containing 10% FBS (Gibco, United States) and 1% penicillin/streptomycin (Gibco, United States) in a 5% CO2 humidified 37C incubator
10.1007/s40618-023-02084-6 J Endocrinol Invest
If hyperglycemia is not controlled during surgery, osmotic diuresis may occur, resulting in excessive loss of fluids and electrolytes
42 (2), e1e12
Experimental design guidance for investigators Model selection and housing Use diet-induced obesity cohorts with pre-randomization by body weight and fat mass, defined sex and age, and thermoneutral housing to decouple thermogenic artifacts
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