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2.3 Cell culture and treatment The human normal hepatic cell line LO2 (Shanghai Institute of Biological Sciences, Chinese Academy of Science, Shanghai, China) was maintained in DMEM(BI,United States) containing 10% FBS (Gibco, United States) and 1% penicillin/streptomycin (Gibco, United States) in a 5% CO2 humidified 37C incubator

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If hyperglycemia is not controlled during surgery, osmotic diuresis may occur, resulting in excessive loss of fluids and electrolytes

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42 (2), e1e12

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Experimental design guidance for investigators Model selection and housing Use diet-induced obesity cohorts with pre-randomization by body weight and fat mass, defined sex and age, and thermoneutral housing to decouple thermogenic artifacts

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