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In vitro GST assay with CDNB (5) Glutathione transferase assays with CDNB ( 5 ) were performed in 50 mM Tris, pH 7.5 with 5% clarified cell lysate, 1 mM CDNB and 1 mM GSH

Cell culture and animal experiments reveal a novel mechanism through which the status of GSH can positively impact the expression of VD metabolism genes

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AB-PAS staining further demonstrated that ATT-I substantially restored the DSS-induced loss of goblet cells, an effect that was diminished upon Erastin co-treatment (Figure 6F)

In the second step, glutathione synthetase (GS), covalently adds the glycine to the -glutamyl-cysteine to produce glutathione