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To quantify the mean florescence intensity (MFI) in each colon section in ImageJ software (NIH, Bethesda, MD), three cross sections for each mouse were analyzed with an optical microscope (magnification, 20)

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pulse on 4 s, pulse off 6 s, total time 40 min), and cell debris were removed by centrifugation (12000 g , 2 min, 4C)

doi: 10.1016/j.heliyon.2023.e19763 202 RodriguezJBystrynJC