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The biodistribution of Cy5-labeled SP and Cy5-labeled SP@M was then tested by in vivo imaging measurement of fluorescence intensity using a Caliper IVIS Lumina III In Vivo Imaging System (Perkin Elmer, USA) at specific time points (0.5, 6, and 24 h post-injection) ( n = 3 in each group)

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A full MS scan was performed at 55825 m / z , with a resolution of 140,000 ppm, in negative ion mode

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