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Briefly, plates were coated overnight with anti-human Fc (Jackson ImmunoResearch Laboratories 109-001-008), blocked for 2 h with PBS 2% BSA at RT, and incubated overnight at 4 C with recombinant Gp1-Fc protein derived from HEK293 cells transfected with LCMV glycoprotein vector

It is important due to the peak of N-Hcy-Lys is eluted close to the biggest peak of reagent sample

Activated ERK1 phosphorylates the threonine 38 residue of ETS1 to induce transcriptional activation [124]

Low B12 levels can cause fatigue, weakness, shortness of breath, headaches, digestive changes, and other symptoms

doi: 10.1038/s41422-022-00642-w 54 NakamuraESatoMYangHMiyagawaFHarasakiMTomitaKet al

This medicine is used fortreating or preventing vitamin deficiency