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Description

Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

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Medically reviewed by Lauren Okafor | MD , The Frank H Netter MD School of Medicine, Loyola University Medical Center on March 16th, 2026

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