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Extracts of the medium were incubated at 80 C for at least 15 min before being centrifuged at 15,000 g for 20 min

Once LCMS analysis was performed, Skyline software was used for metabolite identification

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Yes, but results depend on consistent use and the right dosage

Evaluating dose- and time-dependent effects of vitamin c treatment on a Parkinsons disease fly model

Current perspectives of the roles of the central norepinephrine system in anxiety and depression