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The resulting nanoparticles were purified by centrifugation at 19354 g for 10 minutes (Centurion Scientific Ltd., UK)
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Western blotting Tissue lysates were prepared with radioimmunoprecipitation assay buffer and separated on NuPAGE Bis-Tris Mini Gels (Life Technologies), followed by the transfer to polyvinylidene difluoride membrane (Millipore) following a standard protocol
Furthermore, the expression of p16 ink4a and HMGB-1 in joint cartilage was investigated by immunofluorescence staining to confirm the in vivo elimination of senescent chondrocytes by B2M-CuS NPs
L-GSH supplementation also increased the levels of Th1-associated cytokines, IFN-, TNF-, and IL-2 and decreased levels of IL-6 and IL-10
PMCID: PMC4665094
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