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These behaviors become habitual rather than temporary measures

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After washing the slices with PBS three times, we incubated them with Alexa Fluor 647 goat anti-rabbit secondary antibody (A-21245, Thermo Fisher Scientific, Waltham, MA, USA), diluted 1:500 in the blocking solution, for 2 hours at room temperature

If you cannot keep water down, the risk of electrolyte imbalances increases

Between those points, most people stabilize

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