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The secondary outcomes were insulin, HOMA-IR, HbA1c, GLP-1, Glucagon, total cholesterol, triglycerides, HDL-C, low-density lipoprotein-cholesterol (LDL-C), ALP, AST, ALT, GT, TNF- , IL-6, hsCRP, antioxidant capacity and serum lipid peroxidation, body weight, body mass index (BMI), muscle mass, fat mass, and systolic and diastolic blood pressure Statistical analysis Sample size was established using the SigmaStat32 software, with significance level of 5% and 80% power