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The free glutathione will bind to your GST-tagged protein of interest, freeing it and any interacting proteins from the agarose beads

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To elucidate the mechanism underlying the superior binding, inflamed HUVECs were pre-treated with free anti-vWF antibody for 30 min prior to PLT incubation to block the vWF binding site, resulting in a significant reduction in PLT adhesion

Z., Loizidou, M., Ahmed, M

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(2018) 6:e5203