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If excess free copper were present in a poorly manufactured preparation, it could generate hydroxyl radicals via Fenton-type chemistry and produce artifactual oxidative stress in cell cultures

Targeting ROS in cancer: rationale and strategies

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This hormone is essential for muscle development as it promotes protein synthesis, which is critical for muscle building and maintaining muscle tissue

After being washed with cold PBS, the spheroids were stained with Calcein AM/PI Cell Viability Kit (Beyotime)

Three independent assays were performed to ensure reproducibility