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Complementing immune regulation, Clostridium rebalances gut microbiota homeostasis by enriching beneficial taxa and inhibiting pro-inflammatory bacterial overgrowth and reinforces intestinal barrier integrity by upregulating tight-junction proteins and mucin secretion, thereby blocking the translocation of pathogens and antigens that initiate systemic autoimmunity

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The cells were plated in DMEM containing 10% foetal bovine serum (FBS) and 5.5 mM glucose

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This strain was transformed with p413TEF plasmids with the indicated genetically encoded probes