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For M2 polarization, RAW264.7 cells were stimulated with IL-4 (20 ng mL 1 , ABclonal, China) for 24 h

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Natural GLP-1 is broken down by an enzyme called DPP-4 in a matter of minutes

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Then, once they reach the age of eighty, they should consume enough protein along with the amino acids arginine, lysine, ornithine, and glycine necessary to prevent their IGF-1 level from becoming excessively low