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The presence of bacterial toxins was considered unlikely

Authentic malate or pyruvate samples (Sigma-Aldrich) at a concentration of 10 mM were analyzed accordingly

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Hydrodistension This procedure is done under anaesthesia where the bladder is filled with fluid to evaluate maximum bladder capacity and to treat the pain symptom as well by enlarging the capacity

Assay performance and Validation of NADMED Assay Against Mass-Spectrometry NADMED assays demonstrated high precision with intra-assay coefficients of variation (CV%) ranging from 2.28 to 9.02, and inter-assay precision from 3.93 to 9.37